Active Recombinant Human NTRK1 Protein, His tagged

• Cat.No.: NTRK1-33H
• Product Overview: Active Recombinant Human NTRK1 Protein, Ala34-Gly423, with a C-terminal 6-His tag was expressed in Human embryonic kidney cell, HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 34-423 aa
• Description: This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, cognitive disability and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.
• Form: Long form-TrkA-II. Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.2-1.2 μg/mL in the presence of 10 ng/mL of Recombinant Human beta-NGF.
• Endotoxin: < 0.1 EU/μg by LAL
• Purity: > 95 %, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, ≤ -20 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 1 mg/mL in PBS.
• N-terminal Sequence Analysis: Ala34

Gene Information

• Gene Name: NTRK1 neurotrophic tyrosine kinase, receptor, type 1 [ Homo sapiens (human) ]
• Official Symbol: NTRK1
• Synonyms: NTRK1; neurotrophic tyrosine kinase, receptor, type 1; high affinity nerve growth factor receptor; MTC; TRK; TRKA; gp140trk; Oncogene TRK; tyrosine kinase receptor A; tropomyosin-related kinase A; TRK1-transforming tyrosine kinase protein; TRK1; Trk-A; p140-TrkA; DKFZp781I14186
• Gene ID: 4914
• mRNA Refseq: NM_001007792
• Protein Refseq: NP_001007793
• MIM: 191315
• UniProt ID: P04629