Active Recombinant Human PDCD1 protein, Fc/Avi-tagged, Biotinylated
Cat.No. : | PDCD1-057H |
Product Overview : | Biotinylated Recombinant Human PDCD1(Leu25-Thr168) protein, fused to Fc/Avi tag at the C-terminus, was expressed in CHO cells . |
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Description : | Programmed Death-1 receptor (PDCD1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors (1). Other members of this family include CD28, CTLA-4, ICOS, and BTLA (2-5). Mature mouse PDCD1 consists of a 149 amino acid (aa) extracellular region (ECD) with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The mouse PDCD1 ECD shares 65% aa sequence identity with the human PD-1 ECD. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important for mediating PDCD1 signaling. PDCD1 acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) (6, 7). PD-1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes (8, 9). Ligation of PD-L1 with PD-1 induces co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion (10). Thus, the PD-1:PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance (11). Finally, blockade of the PD-1: PD-L1 interaction by either antibodies or genetic manipulation accelerates tumor eradication and shows potential for improving cancer immunotherapy (12, 13). |
Source : | CHO cells |
Species : | Human |
Tag : | Fc&Avi |
Predicted N Terminal : | Leu25 |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Bio-activity : | Measured by its binding ability in a functional ELISA. When Recombinant Human PDCDL1 Fc Chimera is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human PDCD1 Fc Chimera Avi-tag that produces 50% of the optimal binding response is 10-90 ng/mL. |
Molecular Mass : | 62-88 kDa, under reducing conditions |
Protein length : | Leu25-Thr168 |
Endotoxin : | <0.10 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Applications : | Bioactivity |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 250 μg/mL in PBS. |
Gene Name : | PDCD1 programmed cell death 1 [ Homo sapiens ] |
Official Symbol : | PDCD1 |
Synonyms : | PDCD1; programmed cell death 1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l; |
Gene ID : | 5133 |
mRNA Refseq : | NM_005018 |
Protein Refseq : | NP_005009 |
MIM : | 600244 |
UniProt ID : | Q15116 |
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Not For Human Consumption!
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Customer Reviews (3)
Write a reviewTimely antibody validation, a valuable asset for our laboratory.
Precise protein quantification, fundamental for maintaining data accuracy.
Dependable peptide synthesis, accelerates our peptide-based projects effectively.
Q&As (14)
Ask a questionPDCD1 modulates responses to viral infections, affecting T cell function and viral clearance.
PDCD1 modulates responses to viral infections, affecting T cell function and viral clearance.
PDCD1 helps develop immune tolerance, preventing overactive immune responses.
PDCD1 maintains a balance between immune response and autoimmunity, preventing self-reactivity.
PDCD1 contributes to T cell exhaustion in chronic diseases and cancers, reducing immune efficacy.
PDCD1 maintains a balance between immune response and autoimmunity, preventing self-reactivity.
PDCD1's role in immunotherapy is pivotal, as blocking PD-1 enhances anti-tumor responses.
PDCD1 contributes to T cell exhaustion in chronic diseases and cancers, reducing immune efficacy.
PDCD1 regulates T cell activity by inhibiting immune responses, crucial in immune checkpoints.
PDCD1's role in immunotherapy is pivotal, as blocking PD-1 enhances anti-tumor responses.
PDCD1 helps develop immune tolerance, preventing overactive immune responses.
Changes in PDCD1 signaling impact autoimmune disease progression, influencing immune system activity.
PDCD1 regulates T cell activity by inhibiting immune responses, crucial in immune checkpoints.
Changes in PDCD1 signaling impact autoimmune disease progression, influencing immune system activity.
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