Active Recombinant Human PDCD1 protein, lFc-tagged

• Cat.No.: PDCD1-0614H
• Product Overview: Recombinant Human PDCD1 protein(Leu25-Gln167), fused with lFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: lFc
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human PD-L1, Mouse IgG1 Fc Tag at 0.5 μg/mL (100 μL/well) can bind Human PD-1 Protein, Llama IgG2b Fc Tag with a linear range of 20-313 ng/mL. Immobilized Human PD-1 Protein, Llama IgG2b Fc Tag at 2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.1-2 ng/mL. Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.
• Molecular Mass: This protein carries a llama IgG2b Fc tag at the C-terminus.The protein has a calculated MW of 43.8 kDa. The protein migrates as 55-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu25-Gln167
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PDCD1 programmed cell death 1 [ Homo sapiens ]
• Official Symbol: PDCD1
• Synonyms: PDCD1; programmed cell death 1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l
• Gene ID: 5133
• mRNA Refseq: NM_005018
• Protein Refseq: NP_005009
• MIM: 600244
• UniProt ID: Q15116