Active Recombinant Human PDE8B, GST-tagged

• Cat.No.: PDE8B-1614H
• Product Overview: Recombinant human PDE8B (113-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 113-end
• Description: The protein encoded by this gene is a cyclic nucleotide phosphodiesterase (PDE) that catalyzes the hydrolysis of the second messenger cAMP. The encoded protein, which does not hydrolyze cGMP, is resistant to several PDE inhibitors. Defects in this gene are a cause of autosomal dominant striatal degeneration (ADSD). Several transcript variants encoding different isoforms have been found for this gene.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 40 nmol/min/mg
• Molecular Mass: ~115 kDa
• Purity: >85%
• Applications: PDE Assay
• Stability: 1 yr at -70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: PDE8B phosphodiesterase 8B [ Homo sapiens ]
• Official Symbol: PDE8B
• Synonyms: PDE8B; phosphodiesterase 8B; high affinity cAMP-specific and IBMX-insensitive 3,5-cyclic phosphodiesterase 8B
• Gene ID: 8622
• mRNA Refseq: NM_003719
• Protein Refseq: NP_003710
• MIM: 603390
• UniProt ID: O95263
• Chromosome Location: 5q13.3
• Pathway: G Protein Signaling Pathways; GPCR downstream signaling; Purine metabolism
• Function: 3',5'-cyclic-AMP phosphodiesterase activity; 3',5'-cyclic-nucleotide phosphodiesterase activity; metal ion binding