Active Recombinant Human PDGFA Protein (Ser87-Thr211), GMP Grade, Animal-Free
Cat.No. : | PDGFA-17HG |
Product Overview : | GMP recombinant human PDGF-AA protein (Ser87-Thr211) without tag was expressed in E. coli, produced in an Animal-Free laboratory and manufactured and tested under cGMP guidelines. |
- Specification
- Gene Information
- Related Products
Description : | This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants. |
Source : | E. coli |
Species : | Human |
Form : | Disulfide-linked homodimer |
Bio-activity : | Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is typically 50-200 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin. |
Molecular Mass : | Predicted Molecular Mass: 14 kDa SDS-PAGE: 15-20 kDa, reducing conditions |
Protein Length : | Ser87-Thr211 |
N-terminal Sequence Analysis : | Ser87-Ile-Glu-Glu-Ala-Val-Pro-Ala-Val-(Cys) |
Endotoxin : | <0.01 EU/ μg of the protein by the LAL method. |
Purity : | >97%, by SDS-PAGE with silver staining, under reducing conditions. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, ≤ -20 centigrade under sterile conditions after reconstitution. |
Storage Buffer : | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
Reconstitution : | Reconstitute at 100-200 μg/mL in sterile 4 mM HCl. |
Shipping : | The product is shipped with polar packs. |
Gene Name : | PDGFA platelet-derived growth factor alpha polypeptide [ Homo sapiens ] |
Official Symbol : | PDGFA |
Synonyms : | PDGFA; platelet-derived growth factor alpha polypeptide; platelet-derived growth factor subunit A; PDGF A chain; PDGF A; PDGF1; platelet derived growth factor alpha chain; PDGF-1; PDGF A-chain; PDGF subunit A; platelet-derived growth factor A chain; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha isoform 2 preproprotein; PDGF-A; |
Gene ID : | 5154 |
mRNA Refseq : | NM_002607 |
Protein Refseq : | NP_002598 |
MIM : | 173430 |
UniProt ID : | P04085 |
Products Types
◆ Recombinant Protein | ||
PDGFA-1044C | Active Recombinant Canine PDGFA protein(Ser87-Arg196), hFc-tagged | +Inquiry |
PDGFA-223M | Active Recombinant Mouse PDGFA Protein | +Inquiry |
PDGFA-563H | Recombinant Human PDGFA Protein (Ser87-Thr211), His-tagged | +Inquiry |
PDGFA-1608H | Recombinant Human PDGFA protein, GST-tagged | +Inquiry |
PDGFA-222H | Active Recombinant Human PDGF-AB Protein | +Inquiry |
◆ Lysates | ||
PDGFA-3337HCL | Recombinant Human PDGFA 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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