Active Recombinant Human PGF Protein

• Cat.No.: PIGF-261H
• Product Overview: Recombinant human placenta growth factor-1 produced in insect cells is a homodimer, glycosylated polypeptide chain containing 2 x 131 amino acids. The PLGF-1 is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Description: This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: The ED50 as determined by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. PlGF-1 human Recombinant can bind to immobilized rh-sFlt-1 (100ng/well) with a linear range at 0.5-10ng/ml, corresponding to a Specific Activity of 1x10^5-2x10^6 units/mg.
• Molecular Mass: 34 kDa
• N-terminal Sequence Analysis: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Ala-Val.
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Usage: This products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
• Storage: Lyophilized Placenta Growth Factor 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution PLGF1 should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade.
  Please prevent freeze-thaw cycles.
• Storage Buffer: Lyophilized from a concentrated (1mg/ml) solution containing 50mM acetic acid.
• Reconstitution: It is recommended to reconstitute the lyophilized Placenta Growth Factor 1 in sterile 0.1M acetic acid not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.

Gene Information

• Gene Name: PGF
• Official Symbol: PGF placental growth factor [ Homo sapiens (human) ]
• Synonyms: PGF; placental growth factor; PGFL; PIGF; PLGF; PlGF-2; D12S1900; SHGC-10760; placenta growth factor; placental growth factor, vascular endothelial growth factor-related protein
• Gene ID: 5228
• mRNA Refseq: NM_002632
• Protein Refseq: NP_002623
• MIM: 601121
• UniProt ID: P49763