Active Recombinant Human Platelet Factor 4

• Cat.No.: PF4-194H
• Product Overview: A DNA sequence encoding the human CXCL4 ( NP_002610.1 ) ( Glu 32 - Ser 101 ) was expressed

Specification

• Cat. No.: PF4-194H
• Description: Platelet factor 4 ( PF4 ) is a 70-amino acid protein belonging to the CXC chemokine family also known as chemokine ( C-X-C motif ) ligand 4 ( CXCL4 ) . Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. PF4 is the antigen in heparin-induced thrombocytopenia, an idiosyncratic autoimmune reaction to the administration of the anticoagulant heparin. Its autoantibodies have been found in patients with thrombosis and features resembling HIT but no prior administration of heparin.
• Source: Human
• Host: E.coli
• Form: Lyophilized from sterile PBS , pH 7.4 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
• Purity: > 97 % as determined by SDS-PAGE
• Bio-activity: The ED50 for this effect is typically 4.2 μg/mL; The EC50 of biotinylated human VEGF165 is 9.97 μg/mL
• Endotoxin: ﹤1.0 EU per μg protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70 μ
• Predicted N terminal: Met
• Molecular Mass: The recombinant human CXCL4 comprises 71 amino acids with a predicted molecular mass of 7.8 kDa as estimated in SDS-PAGE under reducing conditions
• Storage: Store it under sterile conditions at -70μ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: PF4 platelet factor 4 [ Homo sapiens ]
• Official Symbol: PF4
• Synonyms: PF4; platelet factor 4; chemokine (C-X-C motif) ligand 4; CXCL4; iroplact ; SCYB4; oncostatin-A; Oncostatin-A; PF-4; Iroplact ; C-X-C motif chemokine 4; Oncostatin-A; MGC138298; Platelet factor 4; OTTHUMP00000160478
• Gene ID: 5196
• mRNA Refseq: NM_002619
• Protein Refseq: NP_002610
• MIM: 173460
• UniProt ID: P02776
• Chromosome Location: 4q12-q21
• Pathway: Chemokine signaling pathway; Cytokine-cytokine receptor interaction; Hemostasis; Signaling by GPCR
• Function: chemokine activity; heparin binding