Active Recombinant Human PRKAA1 /AMPK (A1/B1/G1) Protein, His-tagged

• Cat.No.: PRKAA1-007H
• Product Overview: Active recombinant human C-terminal His-tagged AMPK complex (α1: 1-559; β1: 1-270; γ1: 1-331) was expressed in insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: α1: 1-559; β1: 1-270; γ1: 1-331
• Description: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Bio-activity: Assay performed in Tris-buffered solution containing Mg2+ with 1 mM DTT using AMARA peptide as a substrate (0.1 mg/mL), 20μM ATP and 100μM AMP. Reaction was done at 30 centigrade for 45 min. Amount of ATP transferred was calculated using via luminescent assay.
• Molecular Mass: ~146 kDa (α1: 65 kDa; β1: 38 kDa; γ1: 31 kDa)
• Purity: ≥90% as determined by SDS-PAGE
• Stability: ≥ 2 years
• Storage: At -80 centigrade.
• Storage Buffer: 50 mM sodium phosphate, pH 7.0, with 300 mM sodium chloride, 150 mM imidazole, 0.25 mM DTT, and 25% glycerol

Gene Information

• Gene Name: PRKAA1 protein kinase AMP-activated catalytic subunit alpha 1 [ Homo sapiens (human) ]
• Official Symbol: PRKAA1
• Synonyms: PRKAA1; protein kinase AMP-activated catalytic subunit alpha 1; AMPK; AMPKa1; AMPK alpha 1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; ACACA kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK subunit alpha-1; AMPK, alpha, 1; HMGCR kinase; acetyl-CoA carboxylase kinase; hydroxymethylglutaryl-CoA reductase kinase; protein kinase, AMP-activated, alpha 1 catalytic subunit; tau-protein kinase PRKAA1; EC 2.7.11.1; EC 2.7.11.26; EC 2.7.11.27; EC 2.7.11.31
• Gene ID: 5562
• mRNA Refseq: NM_206907
• Protein Refseq: NP_996790
• MIM: 602739
• UniProt ID: Q13131