Human PRKAA1 Knockdown Cell Lysate

• Cat.No.: PRKAA1-278HKCL
• Product Overview: WB-validated PRKAA1 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 64 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg PRKAA1 KD HeLa cell lysate
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, mTOR signaling pathway, Regulation of autophagy
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: PRKAA1 protein kinase, AMP-activated, alpha 1 catalytic subunit [ Homo sapiens (human) ]
• Official Symbol: PRKAA1
• Synonyms: PRKAA1; protein kinase, AMP-activated, alpha 1 catalytic subunit; 5-AMP-activated protein kinase catalytic subunit alpha-1; AMPK; alpha; 1; AMPKa1; AMPK alpha 1; AMPK subunit alpha-1; tau-protein kinase PRKAA1; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; 5-AMP-activated protein kinase, catalytic alpha-1 chain; MGC33776; MGC57364
• Gene ID: 5562
• mRNA Refseq: NM_006251
• Protein Refseq: NP_006242
• MIM: 602739
• UniProt ID: Q13131