Active Recombinant Human Prolactin Receptor

• Cat.No.: PRLR-4144H
• Product Overview: Extra Cellular Domain Prolactin Receptor Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containsing 210 amino acids and having a molecular mass of 23.97 kDa. The Prolactin Receptor is purified by proprietary chromatographic techniques according to Bignon et al. (1994) JBC 269; 3318-24 and tested according to Gertler et al. (1996) JBC 271; 24482-91.

Specification

• Cat. No.: PRLR-4144H
• Description: The PRL-R consists of at least 3 separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
• Source: Human
• Host: Escherichia Coli
• Form: The Prolactin Receptor was lyophilized from a concentrated (0.4mg/ml) solution with 0.0045mM NaHCO3.
• Purity: Greater than 97.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE. (c) Gel filtration at pH 8 under non denaturative conditions.
• Bio-activity: Activity is determined by the dose-dependant inhibition of Prolactin stimuled proliferation of Nb2 cells and by high affinity binding of ovine Prolactin and other lactogenic hormones in 1:1 molar ratio.
• Physical Appearance: Sterile filtered white lyophilized powder.
• Solubility: It is recommended to reconstitute the lyophilized PRLR in sterile 18M-cm H2O not less than 100µg/ml and not more than 1 mg/ml, which can then be further diluted to other aqueous solutions.
• Amino acid sequence: The sequence of the first six N-terminal amino acids was determined to be Ala-Gly-Lys-Pro-Glu-Ile.
• Protein Content: UV spectroscopy at 280 nm using the absorbency value of 2.63 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Storage: Lyophilized PRL-R although stable at room temperature for 1-2 weeks, should be stored desiccated below -18°C or preferably even at -80°C to prevent dimer formation. Upon reconstitution PRL-R should be stored sterile at 4°C between 2-7 days and for future use below -18°C. For long term storage at 4°C it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles as they cause oligomerization of the protein.

Gene Information

• Gene Name: PRLR prolactin receptor [ Homo sapiens ]
• Official Symbol: PRLR
• Synonyms: PRLR; prolactin receptor; hPRLrI; hPRL receptor; prolactin receptor delta 7/11; secreted prolactin binding protein; delta 4-SF1b truncated prolactin receptor; delta 4-delta 7/11 truncated prolactin receptor; OTTHUMP00000115998; PRL-R; prolactin receptor delta 7/11
• Gene ID: 5618
• mRNA Refseq: NM_000949
• Protein Refseq: NP_000940
• MIM: 176761
• UniProt ID: P16471
• Chromosome Location: 5p13.2
• Pathway: Cytokine-cytokine receptor interaction; Neuroactive ligand-receptor interaction; Jak-STAT signaling pathway; Adipogenesis; ErbB4 signaling events; Ovarian Infertility Genes; Signaling events mediated by PTP1B
• Function: ornithine decarboxylase activator activity; peptide hormone binding; prolactin receptor activity; protein binding; protein homodimerization activity; receptor activity; metal ion binding; cytokine receptor activity