Active Recombinant Human PSCA protein, His-Avi-tagged, Biotinylated

• Cat.No.: PSCA-0630H
• Product Overview: Biotinylated Recombinant Human PSCA protein(Leu12-Ser86), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 12-86 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: 2e5 of anti-PSCA CAR-293 cells were stained with 100 μL of 3 μg/mL of Biotinylated Human PSCA, His,Avitag and negative control protein respectively, washed and then followed by PE-SA antibody and analyzed with FACS.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 12.1 kDa. The protein migrates as 20-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu12-Ser86
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: PSCA prostate stem cell antigen [ Homo sapiens ]
• Official Symbol: PSCA
• Synonyms: PSCA; prostate stem cell antigen; PRO232
• Gene ID: 8000
• mRNA Refseq: NM_005672
• Protein Refseq: NP_005663
• MIM: 602470
• UniProt ID: O43653