Active Recombinant Human PTPN11 Protein, N-terminal 6XHis and AviTag™, Biotinylated

• Cat.No.: PTPN11-18H
• Product Overview: Recombinant SHP2 protein with the N-terminal AviTag™ peptide and His-tag is produced in E. coli cells and site-specifically biotinylated at AviTag with BirA enzyme.

Specification

• Description: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.
• Source: E. coli
• Species: Human
• Tag: The N-terminal 6XHis and AviTag™
• Bio-activity: Specific Activity: ≥100 pmol/min/μg.
• Molecular Mass: ~71 kDa
• Protein Length: 1-593
• Purity: > 80% by Coomassie staining
• Notes: This product is for laboratory research use only.
• Storage: Storage is recommended at -20 centigrade for longer periods of time.
• Concentration: 1.0 mg/mL by A280
• Storage Buffer: 20 mM HEPES, pH 7.5, 100 mM NaCl, 5 mM DTT, 5 mM para-nitrophenylphosphate (PNPP), 30 min at 30 centigrade. Absorbance is measured at 405 nm.
• Shipping: Product requires shipping on ice packs.

Gene Information

• Gene Name: PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ]
• Official Symbol: PTPN11
• Synonyms: PTPN11; protein tyrosine phosphatase non-receptor type 11; CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3; tyrosine-protein phosphatase non-receptor type 11; SH2 domain-containing protein tyrosine phosphatase 2; protein-tyrosine phosphatase 1D; protein-tyrosine phosphatase 2C; EC 3.1.3.48
• Gene ID: 5781
• mRNA Refseq: NM_080601
• Protein Refseq: NP_542168
• MIM: 176876
• UniProt ID: Q06124