Active Recombinant Human/Rat IL23A&IL12B protein, His-tagged

• Cat.No.: IL23A&IL12B-1611H
• Product Overview: Recombinant Human/Rat IL23A&IL12B protein(Arg20-Pro189(IL23A)&Met23-Ser335(IL12B)), fused with His tag, was expressed in HEK293.

Specification

• Species: Human/Rat
• Source: HEK293
• Tag: His
• Protein Length: 20-189;23-335 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human IL-23 R, Fc Tag at 5 μg/mL (100 μL/well) can bind Human IL-23A&Rat IL-12B Heterodimer Protein, His Tag&Tag Free with a linear range of 0.039-0.156 μg/mL.Immobilized Human IL-23A&Rat IL-12B Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Human IL-12 R beta 1, Fc Tag with a linear range of 5-78 ng/mL.
• Molecular Mass: Molecular CharacterizationHuman IL-23A&Rat IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23A and IL-12B, has a calculated MW of 20.6 kDa (IL-23A) and 35.9 kDa (IL-12B). Subunit IL-23A is fused with a polyhistidine tag at the N-terminus and subunit IL-12B contains no tag. The reducing (R) protein migrates as 23 kDa (IL-23A) and 45 kDa and 47 kDa (IL-12B) respectively due to glycosylation.
• Proteinlength: Arg20-Pro189(IL23A)&Met23-Ser335(IL12B)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.