Active Recombinant Human RSPO1 Protein

• Cat.No.: RSPO1-351H
• Product Overview: Active Recombinant Human RSPO1 Protein(NP_001033722.1) (Met 1-Ala 263)was expressed and purified in CHO.

Specification

• Species: Human
• Source: CHO
• Tag: Non
• Protein Length: 1-263 aa
• Form: Lyophilized from sterile PBS, PH 7.4.
• Bio-activity: Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 40-400 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
• Molecular Mass: The recombinant human RSPO1 consists of 243 amino acids and has a predicted molecular mass of 26.8 kDa. As a result of glycosylation, the apparent molecular mass of it is approximately 40 and 31 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: (53.2+43.4) % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_001033722.1
• Unit ID: Q2MKA7
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/Q2MKA7

Gene Information

• Gene Name: RSPO1 R-spondin 1 [ Homo sapiens ]
• Official Symbol: RSPO1
• Synonyms: RSPO1; R-spondin 1; R spondin homolog (Xenopus laevis); R-spondin-1; FLJ40906; RSPONDIN; R-spondin homolog; roof plate-specific spondin-1; RSPO; CRISTIN3; RP11-566C13.1
• Gene ID: 284654
• mRNA Refseq: NM_001038633
• protein Refseq: NP_001033722
• MIM: 609595