Active Recombinant Human SECTM1 protein, hFc&His-tagged

• Cat.No.: SECTM1-1273H
• Product Overview: Active Recombinant Human SECTM1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln29-Gly145) of human SECTM1 (Accession #NP_002995.1) fused with an Fc, 6×His tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: hFc&His
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD7 Protein at 1 μg/mL (100 μL/well) can bind SECTM1 with a linear range of 0.039-1.575 ng/mL.2.Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to SECTM1 coated plates (5 μg/mL and 100 μL/well) in the ppresence of 10μg/ml PHA, approximately 40-50% cells will adhere specifically after 60 minutes at 37°C.
• Molecular Mass: 55 kDa
• Protein Length: Gln29-Gly145
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: > 97% by SDS-PAGE.
• Storage: Store the lyophilized protein at -20°C to -80 °C for long term.
  After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Gene Information

• Gene Name: SECTM1 secreted and transmembrane 1 [ Homo sapiens ]
• Official Symbol: SECTM1
• Synonyms: SECTM1; secreted and transmembrane 1; secreted and transmembrane protein 1; K12; K12 protein; type 1a transmembrane protein
• Gene ID: 6398
• mRNA Refseq: NM_003004
• Protein Refseq: NP_002995
• MIM: 602602
• UniProt ID: Q8WVN6