Active Recombinant Human SENP8 Protein, His-tagged

• Cat.No.: SENP8-829H
• Product Overview: Recombinant Human SENP8 (Accession # NP_660205), fused with a 6-His tag, was produced in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Form: X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 2 mM TCEP
• Bio-activity: Recombinant Human His6-NEDP1/SENP8 is a NEDD8-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-NEDP1/SENP8 concentration of 50-500 nM. A 15 minute pre-incubation with 10 mM DTT is recommended to achieve maximum activity.
• Molecular Mass: Recombinant Human SENP8, His-tagged has a calculated MW of 27 kDa.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SENP8 SUMO/sentrin specific peptidase family member 8 [ Homo sapiens ]
• Official Symbol: SENP8
• Synonyms: SENP8; SUMO/sentrin specific peptidase family member 8; protease, cysteine, 2 (NEDD8 specific) , PRSC2, SUMO/sentrin specific protease family member 8; sentrin-specific protease 8; DEN1; deneddylase 1; HsT17512; NEDD8 specific protease 1; NEDP1; sentrin/SUMO specific protease SENP8; deneddylase-1; protease, cysteine 2; NEDD8-specific protease 1; NEDD8 specific-protease cysteine 2; SUMO sentrin specific protease family member 8; PRSC2
• Gene ID: 123228
• mRNA Refseq: NM_001166340
• Protein Refseq: NP_001159812
• MIM: 608659
• UniProt ID: Q96LD8
• Chromosome Location: 15q22.33