Active Recombinant Human SIRPA protein, hFc&His-tagged
Cat.No. : | SIRPA-2690H |
Product Overview : | Active Recombinant Human SIRP-alpha/CD172a Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu31-Arg370) of human SIRP alpha/CD172a (Accession #NP_542970.1) fused with an Fc, 6×His tag at the C-terminus. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | hFc&His |
Form : | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
Bio-activity : | 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human CD47 at 1 μg/mL (100 μL/well) can bind Recombinant Human SIRP alpha with a linear range of 1-6 ng/mL. 2.Loaded Human SIRP-alpha/CD172a Protein, C-hFc&His on ProA Biosensor, can bind Human CD47 Protein, C-His Tag with an affinity constant of 11.1 nM as determined in BLI assay (Gator). 3.Measured by the ability of the immobilized protein to support the adhesion of the OVCAR-3 cells. The ED50 for this effect is 0.53-2.12 μg/mL. |
Molecular Mass : | 80-100 kDa |
Protein Length : | Glu31-Arg370 |
Endotoxin : | < 0.1 EU/μg of the protein by LAL method. |
Purity : | > 95% by SDS-PAGE. |
Storage : | Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. |
Reconstitution : | Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. |
Gene Name : | SIRPA signal-regulatory protein alpha [ Homo sapiens ] |
Official Symbol : | SIRPA |
Synonyms : | SIRPA; signal-regulatory protein alpha; protein tyrosine phosphatase, non receptor type substrate 1 , PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; BIT; CD172a; MFR; MYD 1; P84; SHPS 1; SHPS1; SIRP; SIRP ALPHA 1; SIRPalpha; SIRPalpha2; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; MYD-1; CD172A; PTPNS1; |
Gene ID : | 140885 |
mRNA Refseq : | NM_001040022 |
Protein Refseq : | NP_001035111 |
MIM : | 602461 |
UniProt ID : | P78324 |
Products Types
◆ Lysates | ||
SIRPA-2298HCL | Recombinant Human SIRPA cell lysate | +Inquiry |
SIRPA-1005RCL | Recombinant Rat SIRPA cell lysate | +Inquiry |
SIRPA-1650MCL | Recombinant Mouse SIRPA cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket