Active Recombinant Human SMPD1 protein, His-tagged

• Cat.No.: SMPD1-537H
• Product Overview: Active Recombinant Human SMPD1 protein(NP_000534.3)(Met1-Pro628) is expressed from Baculovirus-Insect Cells(Sf9) with His tag at the C-terminus.

Specification

• Species: Human
• Source: Insect Cells(Sf9)
• Tag: His
• Protein Length: 1-628 aa
• Form: Supplied as 0.22 μm filtered solution in 20mM Tris, 500mM NaCl, 25% glycerol (pH 7.5).
• Bio-activity: Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is > 1500 pmol/min/μg.
• Molecular Mass: The protein has a predicted MW of 66.53 kDa. Due to glycosylation, the protein migrates to 67-70 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Concentration: 0.2 ug/ul
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_000534.3
• Unit ID: P17405
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/P17405

Gene Information

• Gene Name: SMPD1 sphingomyelin phosphodiesterase 1, acid lysosomal [ Homo sapiens ]
• Official Symbol: SMPD1
• Synonyms: SMPD1; sphingomyelin phosphodiesterase 1, acid lysosomal; sphingomyelin phosphodiesterase; acid sphingomyelinase; ASM; NPD; ASMASE
• Gene ID: 6609
• mRNA Refseq: NM_000543
• protein Refseq: NP_000534
• MIM: 607608