Recombinant Full Length Human SMPD1 Protein, C-Flag-tagged

• Cat.No.: SMPD1-732HFL
• Product Overview: Recombinant Full Length Human SMPD1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 65 kDa
• AA Sequence: MPRYGASLRQSCPRSGREQGQDGTA GAPGLLWMGLVLALALALALALALS DSRVLWAPAEAHPLSPQGHP ARLHRIVPRLRDVFGWGNLTCPICK GLFTAINLGLKKEPNVARVGSVAIK LCNLLKIAPPAVCQSIVHLF EDDMVEVWRRSVLSPSEACGLLLGS TCGHWDIFSSWNISLPTVPKPPPKP PSPPAPGAPVSRILFLTDLH WDHDYLEGTDPDCADPLCCRRGSGL PPASRPGAGYWGEYSKCDLPLRTLE SLLSGLGPAGPFDMVYWTGD IPAHDVWHQTRQDQLRALTTVTALV RKFLGPVPVYPAVGNHESTPVNSFP PPFIEGNHSSRWLYEAMAKA WEPWLPAEALRTLRIGGFYALSPYP GLRLISLNMNFCSRENFWLLINSTD PAGQLQWLVGELQAAEDRGD KVHIIGHIPPGHCLKSWSWNYYRIV ARYENTLAAQFFGHTHVDEFEVFYD EETLSRPLAVAFLAPSATTY IGLNPGYRVYQIDGNYSGSSHVVLD HETYILNLTQANIPGAIPHWQLLYR ARETYGLPNTLPTAWHNLVY RMRGDMQLFQTFWFLYHKGHPPSEP CGTPCRLATLCAQLSARADSPALCR HLMPDGSLPEAQSLWPRPLF
  CTRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome, Metabolic pathways, Sphingolipid metabolism

Gene Information

• Gene Name: SMPD1 sphingomyelin phosphodiesterase 1 [ Homo sapiens (human) ]
• Official Symbol: SMPD1
• Synonyms: ASM; NPD; ASMASE
• Gene ID: 6609
• mRNA Refseq: NM_000543.5
• Protein Refseq: NP_000534.3
• MIM: 607608
• UniProt ID: P17405