Active Recombinant Human SOST protein, His-tagged

• Cat.No.: SOST-870H
• Product Overview: Active Recombinant Human SOST protein(NP_079513.1) (Gln 24-Tyr 213) was expressed in HEK293, fused with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 24-213 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human SOST, His Tag at 1μg/ml(100μl/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 18.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 22.61 kDa. Due to glycosylation, the protein migrates to 32-42 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.
• Publications:
  • Sulfated hyaluronan improves bone regeneration of diabetic rats by binding sclerostin and enhancing osteoblast function (2016)
  • Structural and functional insights into sclerostin-glycosaminoglycan interactions in bone (2015)

Gene Information

• Gene Name: SOST sclerostin [ Homo sapiens ]
• Official Symbol: SOST
• Synonyms: SOST; sclerostin; sclerosteosis; VBCH
• Gene ID: 50964
• mRNA Refseq: NM_025237
• Protein Refseq: NP_079513
• MIM: 605740
• UniProt ID: Q9BQB4

Citation

Sulfated hyaluronan improves bone regeneration of diabetic rats by binding sclerostin and enhancing osteoblast function.

Journal: Biomaterials    PubMed ID: 27131598    Data: 2017/12/18

Authors: Ann-Kristin Picke, Juliane Salbach-Hirsch, Lorenz C Hofbauer

Article Snippet:Fluoraldehyde? ophthaldialdehyde reagent solution was purchased from Thermo Fisher Scientific (Schwerte, Germany).Fluoraldehyde? ophthaldialdehyde reagent solution was purchased from Thermo Fisher Scientific (Schwerte, Germany).. Recombinant human sclerostin was obtained from Creative BioMart (SOST870H, New York, USA) and D-(+) glucose from Sigma-Aldrich (St. Louis, USA).. The Cell Death Detection ELISA and Cell Proliferation ELISA, BrdU were acquired from Roche (Basel, Switzerland) and the Caspase-Glo? 3/7 Assay from Promega (Madison, USA).The Cell Death Detection ELISA and Cell Proliferation ELISA, BrdU were acquired from Roche (Basel, Switzerland) and the Caspase-Glo? 3/7 Assay from Promega (Madison, USA).

Structural and functional insights into sclerostin-glycosaminoglycan interactions in bone.

Journal: Biomaterials    PubMed ID: 26232882    Data: 2016/5/23

Authors: Juliane Salbach-Hirsch, Sergey A Samsonov, Lorenz C Hofbauer

Article Snippet:HA (from Streptococcus, MW? 1.1 106 gmol 1) was purchased from Aqua Biochem (Dessau, Germany), pentylamine-biotin from Thermo Fisher Scientific (Bonn, Germany).HA (from Streptococcus, MW? 1.1 106 gmol 1) was purchased from Aqua Biochem (Dessau, Germany), pentylamine-biotin from Thermo Fisher Scientific (Bonn, Germany).. Recombinant human sclerostin (SOST-870H) was obtained from Creative BioMart (New York, USA). . The Series S Sensor Chips C1, the amine coupling kit and HBS-EP were purchased from GE Healthcare Europe GmbH (Freiburg, Germany).The Series S Sensor Chips C1, the amine coupling kit and HBS-EP were purchased from GE Healthcare Europe GmbH (Freiburg, Germany).