Active Recombinant Human STC1 Protein, His-tagged

• Cat.No.: STC1-01H
• Product Overview: Recombinant Human Stanniocalcin 1/STC-1 Protein (Thr18-Ala247), with a C-terminal 6-His tag, expressed in mouse myeloma cell line.

Specification

• Source: Mouse myeloma cell line
• Species: Human
• Tag: His
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 20-120 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a.
• Molecular Mass: 26-43 kDa
• N-terminal Sequence Analysis: Thr18
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: STC1 stanniocalcin 1 [ Homo sapiens (human) ]
• Official Symbol: STC1
• Synonyms: STC1; stanniocalcin 1; STC; stanniocalcin-1
• Gene ID: 6781
• mRNA Refseq: NM_003155
• Protein Refseq: NP_003146
• MIM: 601185
• UniProt ID: P52823