Active Recombinant Human T-Cell Receptor sCD4 Protein

• Cat.No.: TCRsCD4-143H

Specification

• Species: Human
• Source: Baculovirus
• Tag: Non
• Description: Full length recombinant human T-Cell receptor sCD4 produced in the baculovirus expression system. The full length protein contains V1 through V3 domains of CD4. This protein binds to murine monoclonal antibodies of defined epitope specificity in ELISA and Western ELISA.
• Form: Frozen Liquid
• Bio-activity: This protein binds to native and recombinant HIV-1 gp120 and HIV-2 gp105 and neutralizes HIV-1 infectivity in CD4-bearing, HIV-1 infected cell cultures and HIV infected PCMBs.
• Purity: > 95 % by SDS-PAGE
• Applications: T-Cell Research;
  Human Diagnostics;
  HIV-1 Neutralization;
  Drug Screening.
• Notes: Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
  1. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the secondary detector reagent(s).
  2. Recommended concentration for virus neutralization assays are in 1-10µg/ml of the HIV-1 infected culture.
  3. Drug screening assays are performed with 100-500ng of solid phase sCD4 and gp120 binding is determined in the presence of inhibitors either indirectly using murine anti-gp120-peroxidase conjugate.
  or directly using HIV-1 rgp120-peroxidase conjugate.
• Stability: At least 12 months at -75 centigrade.
• Storage: Store at -75 centigrade.
• Dilutions: PBS