| Species : |
Human |
| Source : |
Insect Cells |
| Tag : |
Flag&His |
| Protein Length : |
1418-2136 aa |
| Description : |
ET (Ten Eleven Translocation) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). 5hmC can be further oxidized into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for initiating the enzymatic deamination process leading to cytosine demethylation. TET1 preferentially binds to CpG-rich sequences at the promoters of both transcriptionally active and Polycomb-repressed genes and modulates gene silencing induced by cytosine methylation by controlling the levels of 5-mC and 5-hmC at gene promoters. TET1 may have the dual function of repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. TET1 is important for stem cell maintenance and specification and is involved in the balance between pluripotency and lineage commitment. TET1 plays an important role in regulating adult hippocampal neurogenesis and cognition |
| Form : |
Recombinant TET1 (1418-2136) protein is supplied at a concentration of 0.2 pg/μL in 25 mM HEPES pH 7.5, 300 mM NaCI, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. |
| Molecular Mass : |
84.3 kDa |
| Purity : |
The purity of the protein is > 75% by SDS-PAGE. |
| Applications : |
Recombinant TET1 (1418-2136) protein is suitable for use in cytosine oxygenase assays, enzyme kinetics, inhibitor screening, and selectivity profiling.
TET Activity Assay Conditions: 250 nM of 30 bp DNA oligos (sequence: 5'-CAGTAACTGTGGTC(mC)GGTAACTGACTTGCA-3', contains a 5mCpG site) was incubated with 100 nM recombinant TET1 (1418-2136) protein in buffer containing 50 mM HEPES, pH 8.0, 100 μM Fe(NH4)2(SO4)2, 2 mM ascorbate, 1 mM alpha-ketoglutarate, 1 mM ATP, 1 mM TCEP at 37 centigrade for 1 hr. Activity was detected by Dot-blot. |
| Storage : |
Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| Concentration : |
0.2 μg/μL |
| Reference : |
1. Huang, Y. et. al.(2019). Cancer Cell. 35(4):677-691. PMID: 30991027. |
