Active Recombinant Human TGFB1 protein

• Cat.No.: TGFB1-120H
• Product Overview: Active Recombinant Human TGFB1 protein(NP_000651.3) (Ala 279-Ser 390) was expressed and purified in CHO. Human, Rhesus and Canine TGFβ1 sequences are identical.

Specification

• Species: Human
• Source: CHO
• Tag: Non
• Protein Length: 279-390 aa
• Form: Lyophilized from sterile 0.1% TFA,26% ACN,pH3.0,5% trehalose, 5% mannitol.
• Bio-activity: Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 0.04-0.2 ng/mL.
• Molecular Mass: The recombinant human/rhesus/canine TGFβ1 consists of 112 amino acids and has a calculated molecular mass of 12.8 kDa. It migrates as an approximately 13 & 26 kDa band in reduced and non-reduced SDS-PAGE respectively, corresponding to the monomer and homodimer.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 88 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  • lncRNA H19 is involved in TGF-β1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway. (2017)
  • lncRNA H19 mediates TGF-β1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway. (2017)
  • Emodin Attenuates Bleomycin-Induced Pulmonary Fibrosis via Anti-Inflammatory and Anti-Oxidative Activities in Rats (2018)

Gene Information

• Gene Name: TGFB1 transforming growth factor, beta 1 [ Homo sapiens ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; DPD1, TGFB; transforming growth factor beta-1; Camurati Engelmann disease; CED; TGFbeta; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide; LAP; DPD1; TGFB
• Gene ID: 7040
• mRNA Refseq: NM_000660
• Protein Refseq: NP_000651
• MIM: 190180
• UniProt ID: P01137