Active Recombinant Human TGFB1 Homodimer

• Cat.No.: TGFB1-051H
• Product Overview: Recombinant human transforming growth factor-β1 is a homodimer containing 224 amino acid residues which is expressed in Dihydrofolate reductase-deficient mammalian cell line CHO/dhfr⁻.

Specification

• Species: Human
• Source: CHO
• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.
• Form: Sterile lyophilized powder
• Molecular Mass: 25.5 kDa
• Bio-activity: Activity is measured by the Mv 1 Lu (NBL-7) cell-inhibition assay, with a specific activity ≥2.5 × 10^7 RU/mg.
• Endotoxin: <1 EU/μg determined by LAL.
• Purity: ≥97%
• Storage: Lyophilized samples remain stable for 36 months when stored at –20 to –70 centigrade from the date of manufacture. After reconstitution, the cytokine can be kept for one month at 2–8 centigrade under sterile conditions, or for three months at –20 to –70 centigrade in a manual-defrost freezer, without significant loss of activity. Avoid repeated freeze-thaw cycles.
• Storage Buffer: This product is supplied as a sterile lyophilized powder in a buffer of 100 mM Glycine, 150 mM NaCl, pH 4.0.
• Reconstitution: Before opening the vial, centrifuge at 10, 000 rpm for 30–60 seconds, or at 3, 000 rpm for 5 minutes, then reconstitute with 0.2 mL of sterile water.

Gene Information

• Gene Name: TGFB1 transforming growth factor, beta 1 [ Homo sapiens (human) ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; DPD1, TGFB; transforming growth factor beta-1; Camurati Engelmann disease; CED; TGFbeta; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide; LAP; DPD1; TGFB
• Gene ID: 7040
• mRNA Refseq: NM_000660
• Protein Refseq: NP_000651
• MIM: 190180
• UniProt ID: P01137