Active Recombinant Human TLR3 protein, His-tagged

• Cat.No.: TLR3-25H
• Product Overview: Recombinant Human TLR3(Lys27-Ser711) fused with His tag at C-terminal was expressed in NS0.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: Lys27-Ser711
• Predicted N Terminal: Lys27
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit poly I:C induced IL-8 secretion by HEK293 human embryonic kidney cells transfected with TLR3. The ED50 for this effect is typically 5-10 µg/mL in the presence of 10 µg/mL of poly I:C.
• Molecular Mass: Predicted Molecular Mass: 79.2 kDa;SDS-PAGE: 110-120 kDa, reducing conditions.
• Endotoxin: <0.1 EU per 1 mug of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 400 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: TLR3 toll-like receptor 3 [ Homo sapiens ]
• Official Symbol: TLR3
• Synonyms: TLR3; toll-like receptor 3; CD283; IIAE2
• Gene ID: 7098
• mRNA Refseq: NM_003265
• Protein Refseq: NP_003256
• MIM: 603029
• UniProt ID: O15455
• Chromosome Location: 4q35
• Pathway: Cytosolic sensors of pathogen-associated DNA, organism-specific biosystem; DAI mediated induction of type I IFNs, organism-specific biosystem; Hepatitis C, organism-specific biosystem; Hepatitis C, conserved biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation, organism-specific biosystem
• Function: double-stranded RNA binding; double-stranded RNA binding; receptor activity; transmembrane signaling receptor activity