Active Recombinant Human TNF

• Cat.No.: TNF-1452H
• Product Overview: Recombinant human TNF-α produced in E.coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 17.4kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 157 a.a.
• Description: Human TNF-α is a 17.4kD factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26kD form of TNF-α is expressed as a membrane bound molecule. TNF-α is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-α have been observed.
• Form: Lyophilized from a 0.2μm filtered solution in 40mM Tris-HCl, 40mM NaCl, containing 0.1% human serum albumin, pH8.0.
• Bio-activity: Specific Activity shall be not less than 2 x10^7 IU/mg.
• Endotoxin: ≤1EU/μg, determined by the LAL method.
• Purity: ≥95%, as determined by SDS-PAGE and HPLC method.
• Usage: FOR RESEARCH ONLY
• Storage: Lyophilized samples are stable for greater than six months from date of receipt at -20 centigrade to -70 centigrade. The reconstituted samples can be stored under sterile conditions at 2-8 centigrade for one month or at -20 centigrade to -70 centigrade for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized rHuTNF-α in sterile ddH2O not less than 100μg/ml, containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution.

Gene Information

• Gene Name: TNF tumor necrosis factor [ Homo sapiens ]
• Official Symbol: TNF
• Synonyms: DIF; TNFA; TNFSF2; TNF-alpha; tumor necrosis factor; APC1 protein; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
• Gene ID: 7124
• mRNA Refseq: NM_000594
• Protein Refseq: NP_000585
• MIM: 191160
• UniProt ID: P01375
• Chromosome Location: 6p21.3
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; Alzheimers Disease, organism-specific biosystem
• Function: cytokine activity; identical protein binding; protease binding