Active Recombinant Human TNFRSF11B protein, His-tagged

• Cat.No.: TNFRSF11B-487H
• Product Overview: Recombinant Human TNFRSF11B protein(NP_002537.3)(Met1-Leu401), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Leu401
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Immobilized Osteoprotegerin Protein, Human, Recombinant (His Tag) at 2 μg/mL (100 μL/well) can bind RANKL Protein, Human, Recombinant (ECD, hFc Tag), the EC50 is 13-64 ng/mL.
• Molecular Mass: The secreted recombinant human TNFRSF11B comprises 391 amino acids and has a predicted molecular mass of 45.3 kDa. As a result of glycosylation, rhTNFRSF11B migrates as an approximately 55 kDa bnd in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  Role of Polymer Architecture on the Activity of Polymer–Protein Conjugates for the Treatment of Accelerated Bone Loss Disorders (2015)

Gene Information

• Gene Name: TNFRSF11B tumor necrosis factor receptor superfamily, member 11b [ Homo sapiens ]
• Official Symbol: TNFRSF11B
• Synonyms: TNFRSF11B; tumor necrosis factor receptor superfamily, member 11b; OPG, osteoprotegerin; tumor necrosis factor receptor superfamily member 11B; OCIF; TR1; osteoprotegerin; osteoclastogenesis inhibitory factor; OPG; MGC29565
• Gene ID: 4982
• mRNA Refseq: NM_002546
• Protein Refseq: NP_002537
• MIM: 602643
• UniProt ID: O00300

Citation

Role of Polymer Architecture on the Activity of Polymer-Protein Conjugates for the Treatment of Accelerated Bone Loss Disorders.

Journal: Biomacromolecules    PubMed ID: 26151628    Data: 2016/5/6

Authors: Bryan S Tucker, Jon D Stewart, Brent S Sumerlin

Article Snippet:Conjugation to OPG.Conjugation to OPG.. Two vials of recombinant human TNFRSF 11B-487 (OPG, 1 mg protein, Creative BioMart, His-tagged, lot #265155) were warmed to room temperature, and each was dissolved in phosphate buffer (250 μL, 100 mM, pH 7.5).. Both solutions were combined and placed in a Millipore Amicon Ultra 0.5 10 000 Da MWCO ultrafiltration unit and centrifuged at 14 000 rpm at room temperature for 10 min to remove interfering excipients.Both solutions were combined and placed in a Millipore Amicon Ultra 0.5 10 000 Da MWCO ultrafiltration unit and centrifuged at 14 000 rpm at room temperature for 10 min to remove interfering excipients.