Active Recombinant Human TNFRSF8 Protein, LIgG2b Fc-tagged, low endotoxin

• Cat.No.: TNFRSF8-373H
• Product Overview: Recombinant Human TNFRSF8 Protein, LIgG2b Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Lys 379 (Accession # NP_001234.2). Predicted N-terminus: Phe 19. This protein carries a llama IgG2b Fc tag at the C-terminus. The protein has a calculated MW of 66.3 kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Species: Human
• Source: HEK293
• Tag: lIgG2b
• Protein Length: Phe 19 - Lys 379
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Recombinant Human TNFRSF8 Protein, LIgG2b Fc-tagged, low endotoxin at 0.5 μg/mL (100 μL/well) can bind Anti-CD30 Mab, Human IgG1 with a linear range of 0.1-1.6 ng/mL (QC tested).
• Molecular Mass: 66.3 kDa
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: TNFRSF8 tumor necrosis factor receptor superfamily, member 8 [ Homo sapiens ]
• Official Symbol: TNFRSF8
• Synonyms: TNFRSF8; tumor necrosis factor receptor superfamily, member 8; CD30, D1S166E; KI 1; Ki-1 antigen; CD30L receptor; CD30; Ki-1; D1S166E
• Gene ID: 943
• mRNA Refseq: NM_001243
• Protein Refseq: NP_001234
• MIM: 153243
• UniProt ID: P28908