Active Recombinant Human TNFSF11 protein, His-Avi-tagged, Biotinylated

• Cat.No.: TNFSF11-0756H
• Product Overview: Biotinylated Recombinant Human TNFSF11 protein(Gly64-Asp245), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human RANK Protein, Mouse IgG2a Fc Tag at 1 μg/mL (100 μL/well) can bind Biotinylated Human TNFSF11 Protein, His,Avitag with a linear range of 0.1-3 ng/mL. Immobilized Human Osteoprotegerin, Fc Tag at 1 μg/mL (100 μL/well) can bind Biotinylated Human TNFSF11 Protein, His,Avitag with a linear range of 0.1-3 ng/mL. Immobilized Anti-TNFSF11 Antibody, Human IgG1 Kappa at 1 μg/mL (100 μL/well) can bind Biotinylated Human TNFSF11 Protein, His,Avitag with a linear range of 0.1-3 ng/mL.
• Molecular Mass: Molecular CharacterizationThis protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag)The protein has a calculated MW of 24.1 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gly64-Asp245
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF11 tumor necrosis factor (ligand) superfamily, member 11 [ Homo sapiens ]
• Official Symbol: TNFSF11
• Synonyms: TNFSF11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11; CD254; ODF; OPGL; RANKL; TRANCE; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; sOdf; OPTB2; hRANKL2
• Gene ID: 8600
• mRNA Refseq: NM_003701
• Protein Refseq: NP_003692
• MIM: 602642
• UniProt ID: O14788