Active Recombinant Monkey TNFSF11 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: TNFSF11-210CAF555
• Product Overview: A DNA sequence encoding the Monkey TNFSF11 (G7PW41) (Gly136-Asp317) (Alexa Fluor 555 conjugated) was expressed with the Fc region of human IgG1 at the N-terminus.

Specification

• Source: HEK293
• Species: Monkey
• Tag: Fc
• Form: Lyophilized
• Bio-activity: 1. Immobilized Cynomolgus S4-Fc3L3-TNFSF11 at 10 μg/mL (100 μL/well) can bind biotinylated human TNFRSF11B-His, The EC50 of biotinylated human TNFRSF11B-His is 7.94-18.52 ng/mL.
  2. The bioactivity of hRANKL was determined by measuring the ability of hRANK
• Molecular Mass: The recombinant cynomolgus TNFSF11 comprises 442 amino acids and has a calculated molecular mass of 48.9 kDa. The apparent molecular mass of the protein is approximately 55 kDa in SDS-PAGE.
• Protein length: Gly136-Asp317， 442
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH7.4.

Gene Information

• Gene Name: TNFSF11tumor necrosis factor (ligand) superfamily, member 11 [Macaca fascicularis(crab-eating macaque) ]
• Official Symbol: TNFSF11
• Synonyms: TNFSF11; tumor necrosis factor (ligand) superfamily, member 11; Receptor activator of nuclear factor kappa-B ligand
• Gene ID: 102137946
• mRNA Refseq: XM_005585744
• Protein Refseq: XP_005585801