Species : |
Human |
Source : |
HEK293 |
Tag : |
Avi&Fc |
Protein Length : |
Ala 105-Leu 250 |
Form : |
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Human BCMA, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human APRIL, Fc,Avitag with a linear range of 1-20 ng/mL.Immobilized Human BCMA, His Tag at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Anti-BCMA MAb (Mouse IgG1, the antibody was co developed by SCT and ACRObiosystems) and 0.05 μg/mL (100 μL/well) Biotinylated Human APRIL, Fc,Avitag with a half maximal inhibitory concentration (IC50) of 4.624 μg/mL.Immobilized Human TACI/TNFRSF13B, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human APRIL, Fc,Avitag with a linear range of 5-78 ng/mL. |
Molecular Mass : |
This protein carries a human IgG1 Fc tag at the N-terminus, followed by an Avi tag.The protein has a calculated MW of 44.6 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. |
Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |