Active Recombinant Human TNFSF13 protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: TNFSF13-2886H
• Product Overview: Biotinylated Recombinant Human TNFSF13 protein(Ala 105-Leu 250), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc-Avi
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human BCMA, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human APRIL, Fc,Avitag with a linear range of 1-20 ng/mL.Immobilized Human BCMA, His Tag at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Anti-BCMA MAb (Mouse IgG1, the antibody was co developed by SCT and ACRObiosystems) and 0.05 μg/mL (100 μL/well) Biotinylated Human APRIL, Fc,Avitag with a half maximal inhibitory concentration (IC50) of 4.624 μg/mL.Immobilized Human TACI/TNFRSF13B, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human APRIL, Fc,Avitag with a linear range of 5-78 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the N-terminus, followed by an Avi tag.The protein has a calculated MW of 44.6 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Ala 105-Leu 250
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF13 tumor necrosis factor (ligand) superfamily, member 13 [ Homo sapiens ]
• Official Symbol: TNFSF13
• Synonyms: TNFSF13; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; APRIL; CD256; a proliferation-inducing ligand; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; TNF- and APOL-related leukocyte expressed ligand 2; TALL2; ZTNF2; TALL-2; TRDL-1; FLJ57090; UNQ383/PRO715
• Gene ID: 8741
• mRNA Refseq: NM_001198622
• Protein Refseq: NP_001185551
• MIM: 604472
• UniProt ID: O75888