Active Recombinant Human UCHL3 protein, His-tagged

• Cat.No.: UCHL3-3090H
• Product Overview: Active Recombinant Human UCHL3 protein(NP_005993.1) (Glu 2-Ala 230) was expressed in E.coli, with a polyhistide tag at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-230 aa
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 20% glycerol, 1mM DTT, pH 8.0.
• Bio-activity: Measured by the hydrolysis of UbiquitinAMC. The specific activity is >14,000 pmoles/min/μg.
• Molecular Mass: The recombinant human UCHL3 consisting of 236 amino acids and has a calculated molecular mass of 27 kDa. It migrates as an approximately 26 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: UCHL3 ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) [ Homo sapiens ]
• Official Symbol: UCHL3
• Synonyms: UCHL3; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L3; ubiquitin thiolesterase; ubiquitin thioesterase L3; UCH-L3
• Gene ID: 7347
• mRNA Refseq: NM_006002
• Protein Refseq: NP_005993
• MIM: 603090
• UniProt ID: P15374