Active Recombinant Human VEGFA

• Cat.No.: VEGFA-703H
• Product Overview: Recombinant Human VEGFA (Accession # NP_001165097) Ala27-Arg191 was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf 21 Insect cells
• Species: Human
• Predicted N Terminal: Ala27
• Form: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323.The ED50 for this effect is typically 1-6 ng/mL.
• Molecular Mass: Recombinant Human VEGFA has a calculated MW of 19.2 kDa (monomer). In SDS-PAGE migrates as 20-22 kDa, reducing conditions39-42 kDa, non-reducing conditions.
• Purity: >97%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. Alternatively, reconstitute at 500 µg/mL in sterile 4 mM HCl containing 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF; vascular permeability factor; VEGF; MVCD1; MGC70609
• Gene ID: 7422
• mRNA Refseq: NM_001025366
• Protein Refseq: NP_001020537
• UniProt ID: P15692
• Chromosome Location: 6p12