Active Recombinant Human VEGFA protein

• Cat.No.: VEGFA-2570H
• Product Overview: Active Recombinant Human VEGF-A/VEGF121(K321N) Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala207-Arg327 (Lys321Asn)) of human VEGF121 (Accession #NP_001020541.2).

Specification

• Source: E. coli
• Species: Human
• Tag: N/A
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human VEGF121 at 2 μg/mL (100 μL/well) can bind Human KDR with a linear range of 0.2-10 ng/mL.
  2.Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.017-0.068 ng/mL.
• Molecular Mass: 18 kDa
• Protein Length: Ala207-Arg327 (Lys321Asn)
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: > 97% by SDS-PAGE.
• Storage: Store the lyophilized protein at -20°C to -80 °C for long term.
  After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF; vascular permeability factor; VEGF; MVCD1; MGC70609
• Gene ID: 7422
• mRNA Refseq: NM_001025366
• Protein Refseq: NP_001020537
• UniProt ID: P15692