Active Recombinant Human VEGFA Protein, His-Avi-tagged, Biotinylated

• Cat.No.: VEGFA-274H
• Product Overview: Biotinylated Human VEGF121, His Tag is expressed from HEK293 cells. It contains AA Ala 27 - Arg 147 (Accession # P15692-9). This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized VEGFR1/R2-Fc at 1ug/mL (100 uL/well) can bind Biotinylated Human VEGF121, His Tag with a linear range of 0.1-2 ug/mL.
• Molecular Mass: The protein has a calculated MW of 16.7 kDa. As a result of glycosylation, the protein migrates as 18 kDa and 22 kDa on a SDS-PAGE gel under reducing condition and 35-40 kDa under non-reducing condition.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE..
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF; vascular permeability factor; VEGF; MVCD1; MGC70609
• Gene ID: 7422
• mRNA Refseq: NM_001025366
• Protein Refseq: NP_001020537
• UniProt ID: P15692