Active Recombinant Human VIM Protein, His-tagged

• Cat.No.: VIM-001H
• Product Overview: Recombinant Human Vimentin Protein (Ser2-Glu466), with a C-terminal 6-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-466 a.a.
• Form: Lyophilized
• Bio-activity: Measured by its ability to bind Recombinant Human NKp46/NCR1 Fc Chimera in a functional ELISA with an estimated KD < 2 nM.
• Molecular Mass: 54.4 kDa
• N-terminal Sequence Analysis: Ser2
• Endotoxin: <0.10 EU per 1 µg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA.
• Reconstitution: Reconstitute at 100 µg/mL in sterile 4 mM HCl.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: VIM vimentin [ Homo sapiens (human) ]
• Official Symbol: VIM
• Synonyms: VIM; vimentin; vimentin; epididymis secretory sperm binding protein
• Gene ID: 7431
• mRNA Refseq: NM_003380
• Protein Refseq: NP_003371
• MIM: 193060
• UniProt ID: P08670