Active Recombinant Human VLDLR protein, His-tagged

• Cat.No.: VLDLR-892H
• Product Overview: Recombinant Human VLDLR protein(NP_003374.3)(Met1-Ser797), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA. 2.Immobilized human VLDLR-His at 10μg/mL (100μL/well) can bind biotinylated human LRPAP1-His, the EC50 of biotinylated human LRPAP1-His is 0.05-0.2 μg/mL.
• Molecular Mass: The recombinant human VLDLR comprises 781 amino acids with a predicted molecular mass of 86 kDa. It migrates with the apparent molecular weight of 150 & 180 kDa due to different glycosylation in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Ser797
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VLDLR very low density lipoprotein receptor [ Homo sapiens ]
• Official Symbol: VLDLR
• Synonyms: VLDLR; very low density lipoprotein receptor; very low-density lipoprotein receptor; VLDL-R; VLDL receptor; CARMQ1; CHRMQ1; VLDLRCH; FLJ35024
• Gene ID: 7436
• mRNA Refseq: NM_001018056
• Protein Refseq: NP_001018066
• MIM: 192977
• UniProt ID: P98155