| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Avi&His |
| Protein Length : |
Phe33-Ala194 |
| Description : |
Platelet receptor Gi24, also known as Dies1, VISTA, SISP1 and B7-H5, is a 55-65 kDa transmembrane glycoprotein with homology to B7-like immune costimulatory molecules (1, 2). Mature human Gi24 contains a 162 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane segment, and a 96 aa cytoplasmic domain. Within the ECD, human Gi24 shares 70% and 67% aa sequence identity with mouse and rat Gi24, respectively (3). The 30 kDa ECD can be shed by MT1-MMP, with a 25-30 kDa fragment remaining in the membrane (3). Gi24 promotes both MT1-MMP expression and the MT1-MMP mediated activation of MMP-2 (3). Gi24 supports the differentiation of embryonic stem cells (ESC) and enhances BMP-4 induced signaling in ESC, but is also down-regulated following BMP-4 exposure (4, 5). It binds to BMP-4 directly, and also associates with the type I BMP receptor Activin RIB/ALK-4 (4, 5). Gi24 is expressed on the surface of naïve CD4+ T cells, regulatory T cells, and adipocytes (6, 7). It is up-regulated in vivo on activated monocytes and dendritic cells (5). Gi24 inhibits CD4+ and CD8+ T cell proliferation, and their production of IL-2 and IFN-γ (6). Its expression on tumor cells attenuates the anti-tumor immune response and enables more rapid tumor progression (6). In contrast, Gi24 limits disease progression in the autoimmune disease model EAE (6). Gi24 also acts as a co-inhibitory receptor on alloreactive T cells to regulate T cell tolerance (8). |
| Predicted N Terminal : |
Phe33 |
| Form : |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Bio-activity : |
Measured by its binding ability in a functional ELISA. When serially diluted Biotinylated Recombinant Human VISTA His-tag Avi-tag Protein is immobilized onto a Streptavidin coated plate, it binds to Recombinant Human VSIG-3 Fc Chimera with an ED50 of 75-450 ng/ml. |
| Molecular Mass : |
35-50 kDa, under reducing conditions |
| Endotoxin : |
<1.0 EU per 1 μg of the protein by the LAL method. |
| Purity : |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Applications : |
Bioactivity |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution : |
Reconstitute at 200 μg/mL in PBS. |
