Active Recombinant Lactobacillus leichmannii Nucleoside deoxyribosyltransferase, His tagged

• Cat.No.: NdT-16L
• Product Overview: NdT02 is a nucleoside deoxyribosyltransferase (type II) from Lactobacillus leichmannii. Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.

Specification

• Species: Lactobacillus leichmannii
• Source: E.coli
• Tag: His
• Description: Type II nucleoside deoxyribosyltransferases catalyze the direct transfer of the 2-deoxyribosyl moiety from a 2′-deoxynucleoside donor to a nucleobase acceptor through a one-step reaction and allow the transfer between purines and/or pyrimidines (Pur↔Pur, Pur↔Pyr, Pyr↔Pyr).
• Form: Liquid, buffered, 50 % (v/v) glycerin
• Molecular Mass: 19.3 kDa
• Storage: At -20 centigrade
• EC No.: 2.4.2.6
• CAS No.: 9026-86-2
• Catalyzed reaction(s): 2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1 Base1 and base2 represent various purines and pyrimidines.
• Example reaction conditions: 50 mM MES pH 6, 1 mM deoxyadenosine, 1 mM uracil, 0.3 g/l NdT02 enzyme, 40 centigrade, 60 min Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
• Other characteristics: Mesophilic, broad substrate spectrum

Gene Information

• Official Symbol: NdT
• Synonyms: NdT; nucleoside 2'-deoxyribosyltransferase type II; NDT; trans-deoxyribosylase; deoxyribose transferase; DRTase; purine(pyrimidine) nucleoside:purine(pyrimidine) deoxyribosyl transferase; nucleoside trans-N-deoxyribosylase; nucleoside:purine(pyrimidine) deoxy-D-ribosyltransferase