| Source : |
HEK293 |
| Species : |
Mink |
| Tag : |
His |
| Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM Nacl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Mink ACE2, His Tag immobilized on CM5 Chip can bind SARS-CoV-2 S protein RBD (Y453F), His Tag with an affinity constant of 0.178 μM as determined in a SPR assay (Biacore T200).Mink ACE2, His Tag immobilized on CM5 Chip can bind SARS-CoV-2 S protein RBD, His Tag with an affinity constant of 8.34 μM as determined in a SPR assay (Biacore T200). |
| Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 86.3 kDa. The protein migrates as 95-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein Length : |
Gln 18-Thr 740 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
