| Species : |
Monkey |
| Source : |
HEK293 |
| Tag : |
Fc |
| Protein Length : |
19-240 aa |
| Description : |
Cluster of Differentiation 86 (CD86) is also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon γ. CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD86 works in tandem with CD80 to prime T cells. Recent study has revealed that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival. Furthermore, the B7 proteins are also involved in innate immune responses by activating NF-κB-signaling pathway in macrophages. CD86 thus is regarded as a promising candidate for immune therapy. CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstline-therapy. |
| Form : |
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant. |
| Bio-activity : |
Immobilized Cynomolgus/Rhesus macaque CTLA-4, His Tag at 2 μg/mL (100 μL/well) can bind Cynomolgus/Rhesus macaque B7-2, Fc Tag with a linear range of 0.039-1.25 μg/mL. |
| Molecular Mass : |
The protein has a calculated MW of 52 kDa. The protein migrates as 76-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Endotoxin : |
Less than 1.0 EU/μg by the LAL method/rFC method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
