Active Recombinant Monkey CTLA4 Protein, Fc-tagged, Alexa Fluor 647 conjugated

• Cat.No.: CTLA4-8852CAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant Monkey CTLA4(Met1-Asp161) fused with Fc region of human IgG1 at C-terminal was expressed in HEK293.

Specification

• Description: CTLA4 played an important role in many functions.
• Source: HEK293
• Species: Monkey
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human B7-1-His at 10 μg/mL (100 μL/well) can bind Cynomolgus CTLA4-Fc, The EC50 of Cynomolgus CTLA4-Fc is 6.0-14.5 ng/mL.
• Molecular Mass: The recombinant cynomolgus/rhesus CTLA4 is a disulfide-linked homodimer. The reduced monomer comprises 366 amino acids and has a calculated molecular mass of 40.4 kDa.The apparent molecular mass of the protein is approximately 52 kDa respectively in SDS-PAGE.
• Protein length: Met1-Asp161， 366
• N-terminal Sequence Analysis: Ala 37
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
• Shipping: Shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: CTLA4 cytotoxic T-lymphocyte associated protein 4 [ Macaca fascicularis ]
• Official Symbol: CTLA4
• Synonyms: CTLA4; cytotoxic T-lymphocyte associated protein 4
• Gene ID: 102115124