Active Recombinant Monkey ERBB3 Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: ERBB3-1179RAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Rhesus (Macaca mulatta) ErbB3 (XP_001113953.2) extracellular domain (Met 1-Thr 643) was fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Monkey
• Tag: His
• Form: Lyophilized
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. Immobilized Rhesus ErbB3 at 2 μg/mL (100 μL/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.58 μg/mL.
• Molecular Mass: The recombinant Rhesus ErbB3 consists of 635 amino acids and predicts a molecular mass of 70.1 kDa. As a result of glycosylation, rhesus ErbB3 migrates as an approximately 100 kDa band in SDS-PAGE under reducing conditions.
• Protein length: 635
• N-terminal Sequence Analysis: Ser 20
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: ERBB3 erb-b2 receptor tyrosine kinase 3 [ Macaca mulatta ]
• Official Symbol: ERBB3
• Synonyms: v-erb-b2 erythroblastic leukemia viral oncogene homolog 3
• Gene ID: 711407