Active Recombinant Mouse ADAM10 Protein, His-tagged

• Cat.No.: Adam10R-10986H
• Product Overview: Mouse ADAM10 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Thr 215 - Glu 673 (Accession # O35598-1). This protein carries a polyhistidine tag at the C-terminus. Predicted N-terminus: Thr 215

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 215-673 aa
• Description: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature enzyme that is involved in the proteolytic release of membrane-bound proteins in a process called ectodomain shedding. Mice lacking the encoded protein die in utero with multiple defects of the developing central nervous system, somites, and cardiovascular system.
• Form: Supplied as 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH7.5 with glycerol as protectant.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2. The specific activity is >5 pmol/min/μg.
• Molecular Mass: The protein has a calculated MW of 52.5 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU/μg by the LAL method/rFC method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: This product is stable after storage at: The product MUST be stored at -70 centigrade or lower upon receipt; -70 centigrade for 3 months under sterile conditions. Please avoid repeated freeze-thaw cycles.
• Concentration: 0.2 mg/mL
• Shipping: This product is supplied and shipped with dry ice, please inquire the shipping cost.

Gene Information

• Gene Name: Adam10 a disintegrin and metallopeptidase domain 10 [ Mus musculus ]
• Official Symbol: ADAM10
• Synonyms: ADAM10; a disintegrin and metallopeptidase domain 10; disintegrin and metalloproteinase domain-containing protein 10; ADAM 10; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10; a disintegrin and metalloprotease domain (ADAM) 10; kuz; MADM; kuzbanian; 1700031C13Rik
• Gene ID: 11487
• mRNA Refseq: NM_007399
• Protein Refseq: NP_031425
• UniProt ID: O35598