Active Recombinant Mouse Alcam protein, His-tagged

• Cat.No.: Alcam-3267M
• Product Overview: Recombinant Mouse Alcam protein(NP_033785.1)(Met1-Lys527), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-527 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Immobilized mouse ALCAM-His at 10 μg/ml (100 μl/well) can bind mouse CD6-Fc, The EC50 of mouse CD6-Fc is 0.08-0.18 μg/ml.
• Molecular Mass: The recombinant mouse ALCAM consists of 511 amino acids and has a predicted molecular mass of 57.7 kDa. Due to glycosylation, rmALCAM migrates as an approximately 70-80 kDa band in SDS-PAGE under reducing conditions.
• Proteinlength: Met1-Lys527
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Alcam activated leukocyte cell adhesion molecule [ Mus musculus ]
• Official Symbol: Alcam
• Synonyms: ALCAM; activated leukocyte cell adhesion molecule; CD166 antigen; BEN; SC1; MuSC; CD166; AI853494; DM-GRASP; MGC27910
• Gene ID: 11658
• mRNA Refseq: NM_009655
• Protein Refseq: NP_033785