Active Recombinant Mouse Anpep protein, His-tagged, Low Endotoxin

• Cat.No.: ANPEP-3347M
• Product Overview: Recombinant Mouse Anpep protein(P97449)(69-966 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 69-966 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >1,000 pmol/min/µg.
• Molecular Mass: The protein has a predicted MW of 103.9 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Anpep alanyl (membrane) aminopeptidase [ Mus musculus ]
• Official Symbol: Anpep
• Synonyms: ANPEP; alanyl (membrane) aminopeptidase; aminopeptidase N; aminopeptidase M; alanyl aminopeptidase; aminopeptidase N/CD13; membrane protein p161; microsomal aminopeptidase; Apn; AP-M; AP-N; Cd13; P150
• Gene ID: 16790
• mRNA Refseq: NM_008486
• Protein Refseq: NP_032512