Active Recombinant Mouse Cd27 Protein, His/Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Cd27-840MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant extracellular domain (Met 1-Arg 182) of mouse CD27 (NP_001028298.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His/Fc
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD27 at 2 μg/mL (100 μL/well) can bind biotinylated human CD70 with a linear range of 0.39-12.5 ng/mL.
• Molecular Mass: The recombinant mouse CD27/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 410 amino acids and has a predicted molecular mass of 46.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD27/Fc monomer is approximately 60-65 kDa due to glycosylation.
• Protein length: 410
• N-terminal Sequence Analysis: Thr 21
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 94 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Cd27 CD27 antigen [ Mus musculus ]
• Official Symbol: Cd27
• Synonyms: CD27; CD27 antigen; CD antigen 27; CD27L receptor; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7; S152; Tp55; Tnfrsf7
• Gene ID: 21940
• mRNA Refseq: NM_001033126
• Protein Refseq: NP_001028298