| Species : |
Mouse |
| Source : |
HEK293 |
| Tag : |
Fc&Flag&His |
| Protein Length : |
Asp 23-Asp 108 (CD3E) & Phe 22-Ala 105 (CD3D) |
| Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100mM Glycine, 25mM Arginine, 150mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Biotinylated CD3e Monoclonal Antibody at 1 μg/mL (100 μL/well) on streptavidin (0.5 μg/well) plate, can bind Mouse CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag with a linear range of 0.016-0.25 μg/mL. |
| Molecular Mass : |
Mouse CD3E&CD3D Heterodimer Protein, Fc, His Tag&Fc, Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 41.5 kDa (CD3E) and 40.7 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag and a polyhistidine tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag and a flag tag at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 55-65 kDa under reducing (R) condition, and 90-116 kDa under non-reducing (NR) condition (SDS-PAGE). |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
