Active Recombinant Mouse CD47 protein, His-tagged

• Cat.No.: CD47-382M
• Product Overview: Recombinant Mouse CD47 protein(Q61735-1)(Gln19-Lys140) is expressed from HEK293 with His tag at the C-terminal.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Mouse SIRP alpha, His Tag immobilized on CM5 Chip can bind Mouse CD47, His Tag with an affinity constant of 1.33 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 14.9 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result.
• Protein length: Gln19-Lys140
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -20 to -80°C for 3-6 months in unopened state after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Cd47 CD47 antigen (Rh-related antigen, integrin-associated signal transducer) [ Mus musculus ]
• Official Symbol: CD47
• Synonyms: CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6
• Gene ID: 16423
• mRNA Refseq: NM_010581
• Protein Refseq: NP_034711
• UniProt ID: Q61735